We provide service to produce IgG-like bispecific antibody from two antibodies which are provided by customers

Based on knobs into holes (KIH) technology for heavy chain heterodimerization, we have developed a method to facilitate correct pairing of cognate heavy and light chains. Our approach replaces the native interchain disulfide bond between the heavy and light chain in one Fab arm with an engineered interchain disulfide bond. The native interchain disulfide bond between the heavy and light chain in another Fab arm is not modified (wild-type). We have identified several different engineered interchain disulfide bonds, which can be chosen to use for the production of IgG-like bispecific antibody.

Our approach can be used to efficiently produce an IgG-like bispecific antibody from two pre-existent antibodies (IgG1, IgG2, IgG3, IgG4, Kappa and Lambda light chains). This approach is easy to operate without using any artificial linkers and common light chains. The resulting IgG-like bispecific antibody is stable and amenable to commercial manufacturing.